Technology is developed from time to time to make human beings more capable of doing new thingsA microscope is also such an instrument that made human beings capable of understanding microorganisms and made them capable of seeing things that cannot be seen from naked eyesThis scientific instrument was first invented in the sixteenth centuryToday there are umpteen microscopes, each having its significance.
Here Are Some Warnings and Mistakes to Avoid as That Will Destroy Your Scanning Electron Microscope
Scanning and Transmission Electron Microscopy
There are mainly two types of electron microscopy, namely – scanning electron microscopy and transmission electron microscopyScanning electron microscopy produces images of the sample by using the scannerElectron beam excites the atoms of secondary electronsIt helps in scanning the topography and also detects the signalsTransmission electron microscopy – as the name suggests, it fires electrons into a thin layer that helps analyze the composition structure of the sample that could be detected from the nature of the collision, the phase of electron beam and its reflection that pierced through the thin layer.
It is advisable to dust and clean the microscope with a soft cloth as otherwise it will slowly pile dust and ruin the microscope and will not deliver the desired results.
Scanning Electron Microscope Resolution
A scanning electron microscope is scanned over the surface of the sample and gives the result of the topography of the surface.SEM is considered the route that can work wonders in generating a high-resolution image of the objectThey help show spatial variation in the composition of chemicals by using the EDS, BSE, and CLThe resolution of the SEM is about 10 nanometers (nm).
Resolution is the soul of the microscope as it helps in imaging the picture clearingThe topography result would be unacceptable if there is a damaged resolution.
How Much Is An Electron Microscope Cost
An electron microscope is prepared with ultimate sophistication, and there are different varieties of the microscope, and each hugely depends on its specificationsThese devices can easily cost thousands to millions of dollarsTherefore, keeping them with high care is crucialThe electron microscope inbuilt many costly devices, such as resolution and magnification power, quality and type of electron detectors, the current and voltage of the electron beams, and high-speed camerasIt also has a vacuum of the specimen chamber, and it is also built according to the type of specimens that will be imaged.
These kinds of highly scientific devices, which are sophisticated and robust, are heavily expensiveIf you find such devices at lower prices, don’t expect the quality and not let you see down to the atomic level.
Comparing the scanning electron microscope and transmission electron microscope, the TEM is more expensive than the otherBut still, the SEM would cost thousands of dollarsIt does not stop here; there are additional expenses of operating and maintenance as well.
Scanning Electron Microscope Principle
SEM plays a vital role in adding kinetic energyThe best thing is that the sample materials can be analyzed repeatedly because the SEM analysis is non-destructiveThe X-rays generated on interactions with electrons do not lead to volume loss.
Components of the scanning electron microscope –
- Display or data output devices
- Electron lenses
- Detectors for all signals of interest
- Electron source
- Sample stage
There are some infrastructure requirements as well like –
- Power supply
- Vibration-free floor
- Vacuum supply
- Cooling system
- A room should be free of electric and magnetic fields.
The Environmental Scanning Electron Microscope
ESEM is the descendant of the conventional Scanning electron microscopeIt is used to image the wet and insulating samplesIt can accommodate the low pressure of gas for around ten tonsThese gases turn into water and then can be maintained in the native stateIt is a unique system where it can examine uncoated biological and industrial materials with an electron beam in a high chamber pressure atmosphere of water vapourThat is why it is capable of analyzing specimens without any destruction and additional procedures.
After knowing about the particular microscope and its maintenance, it is understood that irresponsible behaviour can cost thousands of dollars to buy a new microscope.
Slow Sample Degradation
In some cases, sample degradation may not be visible but in some samples it is prominentIn that case, you can apply some of the techniques to slow down the process of sample degradation as it affects the end result.
The main mistake and disadvantage of SEM is the size and cost of the microscopeIt requires maintenance of voltage, steady current and circulation of cool waterIt is not a child’s play to operate the SEM instead it requires training to operate and prepare the samples.